Applications

Hind Limb Ischaemia

Ischaemic hind limb is a popular experimental model for the investigation of angiogenesis and ischaemia induced neovascularisation. Laser Doppler Imaging can be used to assess global blood perfusion in the hind limb non-invasively and without the use of tracer dyes. This enables the comparison of blood flow in the ischaemic versus non-ischaemic limb. The measurement can be performed over a number of days so that re-perfusion allowed by the development of collateral vessels can be assessed.

Moor Instruments produces a high resolution laser Doppler imager, the moorLDI2-HR, offering fine pixel resolution of 100µm, ideal for imaging angiogenesis.

We recommend the moorLDI2-HR system for use in imaging of angiogenesis.

Contact us for an Application Note (including a detailed method and Practical Suggestions) or to discuss your specific needs for the use of laser Doppler in the assessment of hind limb ischaemia.

References

Aicher A., Heeschen C., Sasaki K., Urbich C., Zeiher A. M., Dimmeler S. 2006 Low-energy shock wave for enhancing recruitment of endothelial progenitor cells: a new modality to increase efficacy of cell therapy in chronic hind limb ischemia. Circulation, 114 (25), pp 2823-30

Crawford R. S., Albadawi H., Atkins M. D., Jones J. E., Yoo H. J., Conrad M. F., Austen Jr W. G., Watkins M. T. 2010 Postischemic poly (ADP-ribose) polymerase (PARP) inhibition reduces ischemia reperfusion injury in a hind-limb ischemia model. Surgery, 148 (1), pp 110 - 118

Tritsaris K., Myren M., Ditlev S. B., Hübschmann M. V., van der Blom I., Hansen A. J., Olsen U. B., Cao R. , Zhang J., Jia T., Wahlberg E., Dissing S., Cao Y. 2007 L-20 is an arteriogenic cytokine that remodels collateral networks and improves functions of ischemic hind limbs. Proc Natl Acad Sci U S A.104 (39), pp 15364-9

Turgeon J., Dussault S., Haddad P., Groleau J., Ménard C, Michaud S. E., Maingrette F., Rivard A. 2010 Probucol and antioxidant vitamins rescue ischemia-induced neovascularization ………. exposed to cigarette smoke: Potential role of endothelial progenitor cells. Atherosclerosis 208, pp 342–349

For additional information on this Application or advice for tailoring Moor Instruments products to your specific application contact us for more information.